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Following primers were used to generate the N419A mutation: forward 5-CCGTCAAGAGATAACGCCAAATGTTATGCAA-3 and reverse 5-TTGCATAACATTTGGCGTTATCTCTTGACGG-3
When it comes to fats, concentrated sources of polyunsaturated fats are not well tolerated at the quantities of fat consumed in a KD, with increased gastrointestinal complaints
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This leaves a substantial population seeking alternatives to surgery
It appears to work well long-term without any serious side effects
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